PCR & qPCR

Q2 Promotion 10% Discount:

Higher Success Rates with Demanding Samples

ALLin™ Hot Start Taq Polymerase & Mastermix

Superior sensitivity polymerase generating  less side products and low copy detection in complex templates with size up to 6 kb.

Highly specific polymerase thanks to efficient blocking at room temperature.

The ALLin™ Buffer includes dNTPs, Magnesium, enhancers and stabilizers.

For maximum convenience, choose the ALLin™ Hot Start Taq  Mastermix with red loading dye for reduced pipetting and minimized errors. 

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ALLin Hot Start Taq Polymerase

High Sensitivity RT-qPCR Detection

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4X 1Step RT qPCR Probe Kits are designed for a sensitive detection of specific RNAs, including virus RNA, in diluted high volume samples. They combine a robust 4X qPCR mix with a 20X
blend of thermostable Reverse Transcriptase and RNase
Inhibitor.

  • Robust 4X qPCR mix for high sample volume input up to 10 µl
  • Detects <5 RNA copies per reaction
  • Successfully tested for Sars-CoV-2 detection
  • Reverse transcription and qPCR in one tube
  • Ideal for multiplex reactions
  • Universal – both standard and fast cycling, GC/AT rich templates
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New Isothermal Amplification Reagents
LAMP, RT-LAMP, WGA, RCA Assays

Isothermal amplification of nucleic acids based on performance of such strand displacement polymerases as phi29 or Bst is a powerful and simple method to detect and amplify low abundance DNA and RNA targets within a short period of time and without the thermal cycler.

  • ALLin™ Isothermal DNA Amplification Kit  is to be used mainly for Isothermal DNA amplification at 70°C, LAMP. Works on DNA templates and includes mix with buffer, dNTPs, Bst Polymerase, dye for optional real-time detection, and water.
  • ALLin™ Isothermal 1Step RNA  Amplification Kit is to be used for Virus RNA detection, SARS-CoV-2 detection, Isothermal  RNA amplification, RT-LAMP. Works on RNA templates and includes Reverse transcription mix, amplification mix with buffer, dNTPs, Bst Polymerase, the dye for optional real-time detection, and water.
  • phi29 DNA Polymerase is a classical recombinant enzyme supplied with 10X buffer and dedicated for use on DNA templates in such applications as isothermal DNA amplification at 30°C, RCA, WGA, MDA and many more.
ALLin Isothermal DNA amplification kit
ALLin 1Step RNA Amplification Kit

Meet the Azure Cielo 3 and Cielo 6 Real-time PCR Systems

The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.

Our novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space.

Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.

Applications

  • Quantitative and qualitative gene expression analysis
  • miRNA analysis
  • Genetic mapping
  • Genetic fingerprinting
  • NGS library quantification
  • 3 or 6 channel multiplex ability
  • Pathogen quantification

Cielo Brochure

Azure Cielo Real-time PCR

Direct PCR of Crude Samples without Template Purification

SampleIN™ Direct PCR Kit

Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading.

Allows direct fast and efficient PCR from various sample types: blood, mouse tail/ear, tissue, buccal swabs, hair follicle, plants.

 

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SampleIN Direct PCR comparison
SampleIN Direct PCR

PCR & qPCR Reagents with Premium Performance

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Contact us about your needs to improve your PCR reactions.

Choose from a range of easy-to-use, fast, highly sensitive and robust polymerases.

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highQu PCR reagents

ALLin™ Mega HiFi DNA Polymerase – the Best Choice for NGS Applications

Highly accurate DNA polymerase for increased sensitivity and great multiplex results.
Fast, high yield PCR with the fidelity 100x higher than Taq.
High thermostability for better denaturation of GC rich templates.
Up to 20 kb long PCR even from complex templates.
Increased processivity for faster amplification and higher yield.

Available as hotstart and non-hotstart.
Get polymerase plus optimised ALLin™ Buffer, or ready-made mastermixes, with or without red dye.

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ALLin Mega HiFi DNA Polymerase

highQu PCR & qPCR Reagents
Performance Excellence

highQu PCR reagents are highly efficient polymerases, available in a flexible range of products depending on application.

Great for challenging templates or crude samples.

Developed for a universal use, fast protocols and high user convenience.

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highQu PCR reagents

1Step RT qPCR Kits for Low Copy Number Virus Detection

The highQu 1Step RT-qPCR kit is successfully used in Covid-19 testing.
With an advanced RNAse inhibitor, an efficient Reverse Transcriptase and a robust qPCR polymerase, this kit is a highly efficient tool for low copy number detection.

<5 copies detected
The 4X mastermix allows higher sample volumes (10 ul sample in a 20 ul reaction) for low viral load detection.
Compatible with all instrument platforms.

1 Step RT qPCR Kits

 

1Step RT-qPCR Kit

SecurRIN™ Advanced RNase Inhibitor

Efficient RNAse inhibition even at higher temperatures up to 60°C.
RNA protection from hydrolysis by RNases A, B and C.
Economical and stable for weeks at ambient temperature.
Robust, works well at 5.5 to 9.0 pH, with 0.5 – 1 mM DTT.
Successfully tested in Sars-CoV-2 detection workflows.
Available in different OEM formats 

SecurRIN RNase Inhibitor Flyer

 

SecuRIN RNAse Inhibitor

highQu PCR Reagents
Excellence in Performance

highQu PCR reagents are highly efficient polymerases.

The products range is flexible depending on application. Great for challenging templates or crude samples.

Developed for a universal use, fast protocols and high user convenience.

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Advice for Successful PCR

Check our video with tips and tricks for your successful PCR testing.

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