NGS, Next Generation Sequencing

Single-Cell Multi-Omics with Tapestri® Technology

Correlations between Protein Expression and Genomic Variants in AML Patients

The Tapestri Platform analyzes genotype and phenotype simultaneously from the same single cells, letting you reveal clonal heterogeneity and target comprehensive biomarkers that help stratify patients more precisely, signal resistance as it begins, and predict relapse.

Check our latest Application Note on the study of single cell genomic variants and protein expression correlations in AML patients.

Application Note

The h/m/r riboPOOLs offer rRNA depletion from human, mouse or rat samples at high or low RNA quality.

Target cytoplasmic rRNA (28S, 18S, 5.8S & 5S rRNA) & mitochondrial rRNA.
Enzyme-free, fast & easy hybridization based workflow.
Any reaction sizes (6, 12, 24, 96, …).
Wide input range (10 ng – 3 μg).
Combination with other ready-made riboPOOLs possible, such as Globin mRNA.

Low quality samples/FFPE:
riboPOOL human/mouse/rat degraded RNA kits
For RIN<7
100% coverage of ribosomal RNA
87-98% rRNA depletion efficiency

High quality samples:
riboPOOL human/mouse/rat kits
RIN>7
97-98% rRNA depletion efficiency

Rib-seq/Ribosome profiling:
riboPOOL ribo-seq human/mouse/rat kitsRibosome Profiling (30-mer contaminants)
100% coverage of rRNA
Adjusted to abundancy of rRNA contaminants
53%-75% rRNA depletion efficiency 

Virus Panel for Multiple Respiratory Pathogen Detection

Celemics Comprehensive Respiratory Virus Panel CRVP

Covering a wide range of respiratory pathogens: 39 different strains from 9 virus types, including SARS-CoV-2, Influenza A/B, RSV A/B and more, the CRVP panel gives a broader picture for not only detection of pathogens but also to study co-infection.

A dedicated bioinformatics data analysis package is included.

Pathogen detection is made at high sensitivity and consensus sequence generation rate down to 100 copies. More than 100X coverage of 99% of viral genome.

The panel was tested using low-quality clinical samples.

Celemics_CRVP Specifications Sheet v1.3

The high complexity riboPOOLs offer efficient removal from a wide range of species.

Multiple-species Pan-riboPOOLs for depletion from similar species:
Pan-Mammal
Pan-Plant
Pan-Bird
Pan-Prokaryote
Pan-Bacteria
Pan-Actinobacteria
Pan-Archaea
Pan-Fungi

Multiple species Combination riboPOOLs for depletion from diverse species groups:
Select from existing riboPOOLs or Pan-riboPOOLs and combine in a ratio reflecting the amount of rRNA from the different species in your sample

Efficient rRNA depletion
Broad coverage of flowering plants
For leaf, seed and root tissue
Targets 28S, 18S, 5.8S & 5S rRNA
Targets mitochondrial rRNA
Targets plastid rRNA

The high abundance of ribosomal RNA (rRNA) limits detection efficiency of relevant RNAs of the transcriptome by RNA-Seq. The Pan-Plant riboPOOL offers a universal solution to selectively deplete cytoplasmic (28S, 18S, 5.8S & 5S), plastid and mitochondrial rRNA of flowering plants in leaf, seed and root tissue.

Broad and Unbiased Pharmacogene Profiling with Targeted NGS

Celemics PharmacoScreen panel encompasses 115 genes related to Pharmacokinetics and Pharmacodynamics

1. Phase I/II drug-metabolizing enzyme genes (drug metabolism)
2. ABC & SLC family transporter genes (drug effect)
3. Pharmacodynamics genes (drug biochemical and physiological mechanism)
4.Modifier genes (drug ADME enhancement). 

The panels feature repetitively optimized probes to capture up to 115 PK/PD-related genes with high coverage (99.6%) and accuracy (99.9%). Sequencing of a Korean cohort (n=376) with the panels enabled profiling of actionable variants as well as rare variants of unknown functional consequences.

Publication

Single-Cell Multi-Omics with Tapestri® Technology

Study Genotype and Phenotype at Single-Cell Resolution

Tapestri workflow provides a great possibility in single-cell DNA sequencing. SNVs, CNVs and protein expression can be studied simultaneously at single cell level.

Panels available for hematology, solid tumour profiling, genome editing, SNVs, SNVs+CNVs, SNVs+CNVs+protein

Video

Rapid and Accurate Covid-19 Sequencing

BT-Seq: Novel Technology for Monitoring and Surveillance

In a collaboration the Korean Centre for Disease Control, Celemics have developed BT-Seq for fast and accurate whole genomce sequencing of small genomes. The technology was used to determine the sequence of Covid-19, isolated in South Korea.

BT-Seq is based on a unique molecular barcode and enzyme technology. Together with a proprietary bioinformatics algorithm, BT-Seq delivers data in short time at the same accuracy as Sanger sequencing.

The technology is currently available as a service and collaboration. A kit will be launched during 2020.

Article

High Fidelity PCR
Fast PCR at Higher Fidelity

The new ALLin™ Mega HiFi DNA Polymerase is the perfect choice for NGS applications.

Fast high yield PCR with fidelity 100x higher than Taq. For amplification also of GC/AT-rich templates.

ALLin Mega HiFi is available in a range of formats:
Hotstart or nont-notstart polymerase
Separate polymerase with buffer including all necessary components
Mastmix with or without red dye

Download protocol

High Uniformity in BRCA Analysis

Hybridisation Capture vs PCR Amplification Enrichment in Targeted NGS

Hybridisation capture enrichment in targeted NGS, using a novel probe design technology, increases data uniformity compared to PCR amplification enrichment. Hybridisation capture gives coverage across UTR´s, Promoters, CDS, Splicing sites and enables detection of more mutants; insertions/deletions, CNV´s, SNV´s and gene fusion.

The unique re-balancing process with full QC will ensure optimal design and efficient workflows.

Download comparison data here!

Target Enrichment Methodology Comparison_Celemics

Simultaneous Ribosomal RNA Depletion in Multiple Species

The riboPOOL™ offers simultaneous ribo-depletion for complex samples. The method will deplete ribosomal RNA simultaneously across multiple species.

Efficient depletion of human, mouse and E. coli RNA with the Combination riboPOOL consisting of a 1:1:1 mix of Human, Mouse/Rat and Pan-Prokaryote riboPOOL was observed for 1 μg RNA input.

RiboPOOLAppnote2019

Microbiomic/Metagenomic Ribosomal RNA Depletion

The Pan-Prokaryote riboPOOL gives the widest microbial coverage for microbiome/metagenomic analysis, covering >75 different species simultaneously. Removing ribosomal RNAs (rRNAs) with high efficiency and targets 5S, 16S and 23S rRNA.

For metagenomics, the riboPOOLs are available as combinational reagent, for example as Pan-prokaryote:Human mix, based on the expected ratios of your sample.

riboPOOLs information page

Efficient, flexible ribosomal RNA depletion for any species

Removing ribosomal RNAs (rRNAs) to allow sensitive detection of scientifically relevant RNAs by Next Generation Sequencing, is currently performed with costly kits limited to well-studied species.
riboPOOLs present an affordable and flexible solution that gives scientists absolute freedom to deplete rRNAs or other custom RNAs from any species.

Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs even outperformed Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.

riboPOOLs information page

riboPOOLs Workflow and Ordering

riboPOOLs are available for any species. Contact us for your specific needs.
riboPOOLs in stock: Human, Mouse, Planarian, Drosophila, Silkworm. Available in 2, 5 or 10 nmol (20, 50, 100 reactions). Custom scales on request.

Protocol is based on biotin-streptavidin chemistry with magnetic bead capture and removal.