Improve Trypsin Digestion & LC-MS Performance with Digestif Quality Control Reagent
Universal Quality Standards
Digestif comprises a soluble recombinant protein scaffold + 11 artificial peptides (iRT) with good ionization.
Amino acids flanking iRT cleavage site either favour or hinder protease cleavage.
Retention time & relative intensity pattern of released iRT can assess the quality of sample workup, the extent of digestion, LC-MS performance & inter/intra-lab variability
SIL Proteins for Quantitative Mass Spectrometry
Low Abundant Protein Quantification in Complex Matrices
The Promise Proteomics SIL proteins are of high purity and >99% isotopic incorporation. You get an internal standard with the same sequence as your target protein of interest, thus normalising for variation in sample digestion and purification processes.
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Choose the right MagReSyn® NTA beads for your his-tagged proteins
Optimise conditions based on the affinity to different chelating metal ions:
Ni2+, Cu2+, Co2+ or Zn2+
The MagReSyn® NTA Screening Kit enables testing of all four alternatives
The MagReSyn® microparticles consist of nitrilotriacetic acid (NTA) residues with a strong metal-chelating property. MagReSyn® NTA is pre-chelated with nickel ions for affinity purification of histidine-tagged proteins. The product has been engineered for exceptional specificity, delivering highly pure target proteins. Since different types of His-tagged proteins may have varying affinities for a particular metal ion, we also offer a His-tagged protein purification screening kit (MagReSyn® NTA-KIT) containing MagReSyn® NTA chelated with 4 alternate divalent metal ions (Ni2+, Cu2+, Co2+, Zn2+) to identify the most suitable metal ion for optimal purification of your particular target protein. MagReSyn® NTA Copper, -Cobalt and -Zinc are currently available in 2
MagReSyn® High-Performance Magnetic Sample Preparation for Proteomics, Molecular- and Cell Biology.
MagReSyn offers high capacity and strong magnetic moment improves the speed of your scientific protocols.
High density functional groups in a hyper-porous polymer matrix enables a maximised binding capacity. The awarded technology also helps to reduce time thanks to the great magnetic features.
Ligands include NTA, TiO2, ZrO2, Streptavidin, Protein A/G, Amine, Carboxyl, Trypsin and Chymotrypsin.