Cell Biology

Non-viral Vaccine Carrier Adjuvants for DNA or Protein Delivery

The new VaxOZ vaccine adjuvants from OZ Bioscience are perfect for any immunization experiment.

Currently, adjuvants remain to be the most important strategy to improve the efficiency of conventional and next-generation vaccines. Genetic adjuvant is an immunological agent uses in combination with a specific antigen in order to boost the immune response. The direct consequence is to produce more antibodies and longer-lasting immunity than with the antigen alone and thus minimizing the dose of antigen needed to the vaccine. In particular, DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) based cationic lipids induce maturation of dentritic cells, which then traffic to the draining lymph nodes to activate antigen-specific T cells.

In vivo grade manufactured and quality controlled, the VaxOZ adjuvants are intended for research purposes only.

 

 

CaLiVax DOTAP cationic lipid-based composition for liposome-mediated DNA or protein vaccines.

PolyVax-CPO Vaccine Adjuvant is a cationic polymer genetic adjuvant that associates with plasmid DNA to form an efficient polymer-based nanoparticle delivery system (NPD).

CNE-CPO (Cationic Nano Emulsion) Vaccine Adjuvant is an oil-in-water Cationic Nano Emulsion made of squalene droplets and cationic polymers in a continuous aqueous phase. CNE-CPO Vaccine Adjuvant is biodegradable, an important advantage over alternative oils that have been used in emulsion adjuvants.

Enhancing Cell or Nuclei Recovery in Single Cell Multi-omics

Single-cell genomics and proteogenomics workflows can be greatly improved by the Laminar Wash™ technology.

The Laminar Mini is the perfect tool for single-cell sequencing experiments. It washes 8 samples simultaneously in a few minutes. It replaces centrifugation and preserves precious samples at low cell numbers. The workflow is easier and faster.

Laminar Mini

In the recent webinar, Curiox, Biolegend and ImmunAI presented data on increased cell retention compared to centrifugation, higher signal-to-noise ratios and time savings.

View webinar for single-cell multi-omics

 

Automatic Live Cell Imaging System: Celloger Mini

Celloger Mini is a compact and easy-to-use live cell imaging system.

Compact design and easily fits in CO2 incubators
Positioning multiple area up to 96 well
Time-lapse image capturing & video clip
Convenient user software to operate the system
Providing the thermo-hygrometer data in real time 

Celloger Mini

 

Automatic, Accurate and User-Friendly Cell Counter FACSCOPE B

FACSCOPE B has unique propoerties for rare cell populations and does not require any manual focusing.

Accurate and fast; choose between three different modes: Quick, Normal and Precise
Rare populations: count rare cells in the precise mode, allowing up to 3,6 µl volume
Convenient to use: automatic focusing

 

FACSCOPE B Specifications

 

Enhance Lentiviral Transduction in Stem- and Immune Cells using LentiBlast Premium

The Lentiblast Premium Transduction Enhancer is designed to enhance viral transduction in many types of primary cells – adherent and suspension, especially also in CD34+ hematopoietic stem cells or T-lymphocytes. The image shows data on the effect of using LentiBlast Premium in CD34+ stem cells at a low viral particle/cell number ratio.

LentiBlast Premium is a patented reagent from OZ Biosciences with the following key benefits:
Higher efficiency in hard-to-transduct cells
High viability; non-toxic reagent
Save effort and money; use lower viral titers
Easy to use protocol

 Tips & tricks for successful viral transduction

 

Magnetofection™ for Enhanced Transfection Efficiency in Primary and Hard-to-Transfect Cells

Magnetic Assisted Transfection is a novel technology developed by OZ Biosciences. It has a proven high efficiency in delivering molecules into challenging cells. 
Magnetic nanoparticles are associated with nucleic acids (naked or pre-complexed with a transfectionreagent or viruses) by salt-induced aggregation and electrostatic interactions.
Magnetic force drives these complexes towards the target cells, allowing a rapid concentration of the vector dose onto cells
The cellular uptake of the genetic materials is accomplished by endocytosis and pinocytosis
Nucleic acids are released in the cytoplasm by flip-flop mechanism or proton sponge effect*

Magnetofection data

 

* Plank et al , Adv. Drug Deliv. Rev. (2011), 63(14-15):1300-31

 

New Transfection and Transduction Reagents with Unique Technologies

 

The reagents from OZ Biosciences covers a large range of applications:
Transfection Reagents
Viral Applications
Genome Editing
Protein Delivery
In vivo Delivery
Cellular Assays
Vaccine Adjuvants
Transfection Tools

Get catalogue

 

Laminar Wash Sample Preparation for Single-Cell Sequencing

Single-cell genomics is rapidly growing. Nature Methods named multimodal omics the method of the year for 2019. And as more scientists use single-cell techniques, they find that sample preparation is very important to achieve high-quality data.

Single cell sequencing can suffer from poor data quality due to clumping of cells, incomplete washing of cells leaving cell-free nucleic acids, and poor retention of low numbers of cells. Washing via centrifugation causes stress on the cells in samples and can cause erroneous gene expression results.

Sample prep is extremely important in single-cell genomics, especially for single cell gene expression and immune profiling. In this area, mRNA transcripts are being barcoded and cells can experience mRNA leakage (causing increased background) or mRNA degradation (causing less signal). Unlike with flow cytometry, in single-cell sequencing it is not possible to gate out debris or groups of cells. The cell preparation must be as clean as possible and consist of single cells.

By using the Laminar Wash system scientists can see time savings and less manual manipulation.

Laminar Wash systems can wash cells without putting stress on cells that may impact gene expression, cell integrity and viability.

Laminar Wash™ Technology Overview

 

Nuclei Isolation Using Laminar Wash

PBMCs were lysed in bulk in tube with Lysis Buffer, quenched with Wash Buffer and centrifuged
Cells are further either washed twiced in tube by centrifuge, OR plated in Laminar Wash plate to settle for 20min, washed 7x in a Laminar Wash HT1000 System with PBS + 0.04% BSA

CAR-T-Cell Production

Reliable, Automated and Reproducible Flow Cytometry

The unique Laminar Wash™ technology simplifies and automates flow cytometry workflows for CAR-T-cell manufacturing. Data improves reproducibility, saves time and gives tighter populations. Laminar Wash™ replaces centrifugation-based stain&wash and is more gentle to cells. Time saving in this workflow was 50 min/assay.

CAR-T cell therapy biotech company AdiCet Bio improved data by automating of a part of their flow cytometry assay. They saw improved consistency and are planning full automation using a Laminar Wash™ system.

Download AdiCet Bio Case Study

Laminar Wash™ Technology Overview

 

Change the way you process cells to get reliably consistent data in flow cytometry

The DA-Cell Laminar wash technology replaces centrifugation by using a novel DA-Cell technology, which is gentle to cells, giving higher retention.

  • A wide range of cell numbers, from single-cell to 10 million cells per well
  • No stress on cells from centrifugation and improved viability
  • Reduced debris and aggregation of cells
  • Cleaner and faster washing

DA-Cell technology page

Enhanced viability and retention

Cells from DA Cell method demonstrate much stronger fluorescence signal and intact morphology of cells

Centrifugation presumably causes  significant stress on cells distorting its physiology and protein expression

This is how it works!

Check our video describing the efficient laminar wash of suspension cells for flow cytometry

siRNA Off-Target Effects; Causes, Extent & Impact
siPOOLs™ are innovative, complex siRNA pools which reduce false positives & false negatives in siRNA knockdown experiments

Screen Shot 2017-10-03 at 16.30.27

Individual siRNAs or low complexity siRNA pools containing 3-4 siRNAs often hit multiple off-target genes and exhibit variable target gene knock-down. Short interfering RNA (siRNA) pools, siPOOLs are highly complex and defined pools of 30 siRNAs, each siRNA present at picomolar working concentrations.

Benefits:
dilutes the off-target signature of each siRNA, increasing on-target specificity
ensures co-operative knock-down of the target gene, producing more robust and reliable results.

Download the new TechNote:
Off-Target Effects; Causes, Extent & Impact

Publication:
Hannus et al NAR

 

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